The Lion Bee Gate, aka the Mountain Top Bee Gate, is a gate that requires 25 bees to pass through.
It is given its name because of the large Lion Bee on it.
Before the update, (4/27/2018) there was a method to bypass the Lion Bee Gate. without the 25 Bees (using the parachute); this method has since been patched. One can also bypass the gate if they have less than 25 bees in their hive by having discovered 22 different bee types, and using the red cannon. (walking through the gate to exit will kill you)
One can also bypass the gate currently if they have less than 25 bees in their hive by having discovered 12 different bee types and a parachute, and using the yellow cannon. (Same, walking through the gate to exit will kill you)
Watch the video from 0:53, the other part is all intros
The Lion Bee Gate gives you access to the following locations: